Today we are the most uniquely diversified independent contract testing laboratory network in the world. Instruments used in microbiology laboratory with principle and uses. In microbiology laboratory, horizontal ty pe of laminar air flow is used to supply the air through f ilter. Introduction to microbiology laboratory and its equipments. Oct 04, 2019 whether youre studying microbiology in high school or as a college student, youll need to write a number of lab reports. It also insures culture media contain only the bacterium you are studying. Common laboratory techniques in all volumetric glassware pipet, buret, volumetric flasks, graduated cylinder, etc. Which of the following types of personal protective equipment ppe is frequently used. The goal of the laboratory is to expose students to the wide variety of lives in the microbial world. Transitional rooms at the rear ofthe laboratory are needed for receiving laboratory and animal. Laboratory manual and workbook in microbiology applications to patient care 7th edition josephine a. Laboratory coats should fit properly and should cover arms to the wrist. Basic equipment and microbiological techniques for studying. Aseptic areas, sterile room, the demand independence.
Some of the most important items of equipment are the following. Demonstration of capability doc, initial and ongoing 2. Throughout this manual, the student will learn the scientific method and its application. Planning a food microbiology laboratory lab furniture. Autocla ves are used to sterilize equipment and supplies b y subjecting them to high pressure saturated steam at 1 2 1c f or around 1 520mins depending on the size of the. The final portion of this manual involves the identification. Transitional rooms at the rear ofthe laboratory are needed for receiving laboratory and animal room equipment and supplies and for removing equipment, trash, and other items from the infectious areas. List of equipment apparatus used in microbiology laboratory 1. Spaces between benches, cabinets, and equipment must be kept accessible for cleaning. The laboratory has sufficient quantities of equipment and supplies to carry out the volume of work. Mar 28, 2019 easy to adapt for almost any microbiology lab course, this versatile, comprehensive, and clearly written manual is competitively priced and can be paired with any undergraduate microbiology text.
Laboratory doors must be locked when the laboratory is not occupied. Print lab equipment names and pictures quizlet quizlet. Pdf an assignment of microbiology laboratory use of. Microbiology lab 10 working with microorganisms can pose a risk to both the laboratory staff and the outside environment, including production.
Microbiology laboratory safety rules and procedure learn. Instruments used specially in microbiology include. Jun 17, 2020 an analytical balance is a type of balance that is commonly used for the measurement of mass in the submilligram range. Typically, this mayinclude a small throughthewall ethylene oxide gas cham ber for thecold sterilization ofheatsensitive ma terials 8 and a uv apparatus for decon taminating sheets ofpaper passed out ofthe in fectious area, as illustrated in fig. Strategies that keep the experiment aseptic include flaming the. Bacterial adhesion personal protective equipment ureteral stents basic equipment. Microbiology module laboratory safety and standards precautions microbiology 68 notes sheets msdss before moving, handling or opening chemicals.
Basic information on qualification requirements, documentation, etc. Some of the glassware instruments used in microbiology laboratory are. This manual has been written for a righthanded person. Instruments specifically used in microbiology include. Third, general instruments and equipment a specific lab instruments and equipment according to the requirements of your choice 1. To isolate and study microorganisms in pure culture, the microbiologist requires basic laborato ry apparatus and the application of specific techniques, as. Bunsen burner bunsen burner is a common tool used in science lab fig. People must be advised of potential hazards before entering the work area. Equipments used in microbiology laboratory pdf squarespace. Easy to adapt for almost any microbiology lab course, this versatile, comprehensive, and clearly written manual is competitively priced and can be paired with any undergraduate microbiology text. Microbiology equipment is a large category covering all kinds of items used in microbiology laboratories. The three main elements of safe containment of microorganisms are 1 good laboratory practices and technique, 2 safety equipment, and 3 facility design.
Instruments used in microbiology lab with principle and uses. Introduction to laboratory rules and procedures, laboratory equipment and apparatus the first session of this practical will be a demonstration and the class will be divided into four groups, each of which will visit the following stations. A microbiology lab assistant renders support to the scientists and researchers at the microbiology laboratory. Gsc equipment catalog 2019 center for galapagos studies. They are used to heat on hold reagents for observing. In addition to microorganisms, there are some chemicals used in this laboratory that are potentially harmful. Microbiology lab assistant resume samples qwikresume. Basic practical microbiology why microbiology matters. There should be a documented programme for the qualification, calibration, performance verification, maintenance, and a system for monitoring the use of its equipment.
What are the critical elements in the microbiology laboratory. Absolutely no food, drinks, chewing gum, or smoking is allowed in the laboratory. Microbiology laboratory manual prepared by abdelraouf elmanama. Lab a te i g emsls network of nationwide laboratories has been providing quality analytical services since 1981. Laboratory doors should be kept closed and access to laboratory areas must be limited and controlled. Microbiology methods for wastewater water laboratories. This manual serves as a general introduction to the microbiology laboratory, including basic procedures and equipment. An analytical balance is a type of balance that is commonly used for the measurement of mass in the submilligram range. Annex 2 who good practices for pharmaceutical microbiology. Never use a product from an unlabeled container, and report missing labels to your supervisor. Microbiology a laboratory manual 11th edition 2016 pdf. All doors leading into and out of the student laboratories must be closed at all times.
This book covers the basic and clinical facet of bacteriology, mycology, parasitology, virology and immunology. Read the overview below and download using the links given at the end of the post. Also learn about the process of handling and caring of laboratory glasswares. The competency of microbiology professionals is obtained through the theoretical knowledge gained in the class and the practical skills and applications that lead to the better understanding and interpretations the subject matter. Protective clothing and equipment laboratory coats protective coats, gowns, smocks, or uniforms designated for laboratory use must be worn while working in the laboratory.
Aseptic technique is a combination of laboratory methods that keep the worker and work area free of contamination. Mar 16, 2017 image result for microbiology laboratory equipments and their uses pdf. The manual covers lab safety, how to use a microscope, aseptic transfer of bacteria, simple, gram, capsule, acid fast, spore, and negative staining, environmental requirements of bacteria, antibiotic susceptibility testing, bacterial control methods, membrane. Children under 12 years of age and pets are not allowed in laboratory. Put off the shoes before entering to operate the apparatus. Pdf on oct 10, 2008, abu nayeem published an assignment of microbiology laboratory use of microbiology lab, chemical and equipment. These types of balances are made with a measuring pan enclosed in a transparent covering that prevents smalls particles or air currents from getting collected on the pan. General microbiology laboratory manual molbmicr 2021 fall semester 2010 university of wyoming laramie, wyoming. Biohazard signs or stickers should be posted near all laboratory doors and on all equipment used for laboratory work e. Be able to identify the name of each piece of equipment, as well as its function or use in the laboratory. Laboratory equipment and supplies refrigerator maintain temperature for samples of 0.
Usually this meniscus curves downward to a minimum at the center. The experiments to be conducted in the general microbiology laboratory are outlined in this manual. Eyewash must be flushed at least monthly and kept clear of obstructions. We will be working with a variety of materials that can cause. Essential duties and responsibilities that are seen on the microbiology lab assistant resume include the following assisting scientists who study and research on organisms such as fungi, viruses, algae, and bacteria. Enumeration of microbial population in soil bacteria, fungi, actinomycetes. Common laboratory apparatus beakers are useful as a reaction container or to hold liquid or solid samples. Name picture use ring stand supports the bunsen burner, iron ring, pipestem triangle, and other items, often while heating a substance. The most important glassware used in a microbiological. Read the manual carefully before using the instrument.
Safety in a microbiology laboratory is important in the prevention of infection as microbiology laboratory cultures, manipulates, and uses virulent andor potentially pathogenic microorganisms. Laboratory equipment and work surfaces should be decontaminated with an appropriate disinfectant on a routine basis, and especially after spills, splashes, or other contamination. In microbiology, these items can include, but are not limited to. This section was originally included more for the sake of completeness than because of concerns peculiar to the microbiology laboratory. The organization reflects the body systems approach and contains specific sections on clinical diagnosis. Glassware and instruments used in microbiology laboratory. General microbiology laboratory manual by rachel m watson. They are also used to catch liquids from titrations and filtrates from filtering operations. Basic microbiology laboratory for upper division college students with color diagrams and very simple instructions.
An introduction to microbiology, aseptic technique and safety preparation safety guidelines 1 risk assessment 2 good microbiological laboratory practice gmlp 3 spillage management 3 aerosols 3 resources equipment 4 apparatus 5 materials 5 media, sterilisation and disinfection preparation of culture media 6 pouring a plate 6 storage of media 6. List of equipment apparatus used in microbiology laboratory. The equipment is electrically powered and uses ammonia as the refrigerant. This book covers the basic and clinical facet of bacteriology, mycology, parasitology, virology and. A liquid in a smalldiameter container will form a meniscus or curve at the surface of the liquid. It is used for sterilization of glasswares, such as test tubes, pipettes. This protective clothing should be removed and left in the laboratory before leaving for non. Microbiology lab safety university of minnesota duluth. All materials that have come in contact with a microbial organism. A safety data sheet contains information about firstaid. Known for its thorough coverage, straightforward procedures, and minimal equipment requirements, the eleventh edition incorporates current safety. Burets are for addition of a precise volume of liquid. A balance is used to find out the mass of a substance by comparing it with known masses.
Instruments used specially in microbiology include instrument list. Pdf veterinary microbiology laboratory manual sultan a. The lab report genre does have several sections youll need to meet in your report, which include. Microscope parts, principles of microscopy, resolving power and numerical aperture. Additional protocols and equipment lists are outlined below. Wash your hands with disinfectant soap when you arrive at the lab and again before you leave. June 2002 overview this microbiology laboratory manual is designed especially for the nonmajors, health science.
Equipment microbiology laboratories should be appropriately equipped to perform all of the activities undertaken. Laboratory equipment used in the microbiology laboratory should not be used outside the microbiology area, unless there are speci. This microbiology laboratory manual is designed especially for the nonmajors, health science microbiology courses. Standard safety practices in the microbiology laboratory.
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