Ms is one of gcthe best techniques to identify the bioactive constituents of the plant extracts. At that time there was no synthetic medicines, they have been using only the herbal. Preliminary phytochemical screening, antibacterial potential. Department of biochemistry 2, avinashilingam institute of home science and higher education for. Gcms analysis, phyllodium pulchellum, ethanol leaf extract, bioactive compounds. The present investigation was carried out to determine the possible bioactive components of leaves and bark of f. In olden days itself the importance of medicinal plants have been discovered. The results pertaining to gcms analysis led to the identification of number compounds from the gc fractionations of the ethanol extract of polyherbal drug. Gcms analysis of these extracts were performed using a perkinelmer gc clarus 500 system and gas chromatograph interfaced to a mass spectrometer gcms equipped with a elitei, fused silica capillary column 30mmx0. The chemical compositions of methanol extract of plant. Phytochemical investigation and antioxidant activity of. Annapoorani 2 department of biochemistry 1, sacred heart college autonomous, tirupattur 635601, tamil nadu, india.
Crude powder of plant used for ftir analysis and ethanolic extracts was analyzed using gcms. Two methods were developed for the quantitative analysis of phenolic acids in herb extracts. Antioxidant activity and determination of bioactive compounds. The extracts used for phytochemical tests were concentrated by evaporation and stored at 4c and used for gcms analysis. The changeable volatile compounds profiling of methanol extract from mangosteen leaves was measured using gcms. Gcms analysis of bioactive compounds in methanolic extract of. Gcms analysis was carried out on a gc clarus 500 perkinelmer system comprising a gas chromatograph interfaced to a mass spectrometer gcms instrument employing the following conditions. Phytochemical screening of plant extracts and gcms analysis of n. Gcms analysis of petroleum ether extract of alysicarpus. Eclipta alba has an important role in the traditional ayurvedic, siddha and unani systems of medicine. By gas chromatographymass spectrometry gcms analysis 3allylguaiacol 65. Conducted lcms analysis revealed the presence of 17 compounds in flowers and leaves extract with the main were quinic acid and naringin followed by rutin, hyperoside, gallic acid and apegenin7oglucoside.
Employing fingerprinting of medicinal plants by means of lc. The methods were compared in terms of their linearity, repeatabil. The presence study was aimed to catalyze the primary metabolites and their confirmation by using gcms analysis and antibacterial potential of leaf extract of two important medicinal plant viz. Gcms analysis and insilico antipsychotic activity of. Exploration of medicinal plants for curative purposes is mainly based on the available traditional information from the experts and local population 5, 6. In vitro pharmacological activities and gcms analysis of different.
The extracts and fractions were analysed chemically using gcms, and their ability to inhibit platelet aggregation was investigated. Mainly on traditional remedies such as herbs for their history it has been used as a popular folk medicine. Hence gcms analysis was performed on methanol extract. At present, about 25% of the active constituents have been identified from medicinal plants, which have been used as prescribed medicines. Gcms analysis of phytochemical constituents in methanol. Gc ms analysis of methanolic extract of erythrina variegatahemoglobin, gc l.
The name and specification of the column used is rtx5ms 30m x 0. Aug 09, 2011 hplc and gcms determination of bioactive compounds in microwave obtained extracts of three varieties of labisia pumila benth. The crude extract was prepared from the whole plant powder of s. Gas chromatography mass spectroscopy analysis gcmsa gcms study was performed at indian institute of crop processing technology iicpt thanjavur, to study the phytochemical components present in the methanol extract of wheatgrass. Oct 02, 2020 the gcms analysis of ethyl acetate and methanol extracts from leaves, and methanol extract from rhizomes of a. Pdf different types of herbs often used in pharmaceutical, cosmetic and food industry. This technique is best for identification of various phytochemicals of plant. Phytochemical screening and gcms analysis of acetone leaf.
Flowers and leaves extracts showed a powerful antimicrobial effect against all strains and the most observed effect. This is first time reporting of gcms analysis of nhexane extract of r. The antibacterial potential of the methanol leaf extract of the studied species was tested against escherichia coli, pseudomonas aeruginosa, klebsiellap neumoniae, streptococcus pyogens, staphylococcus aureus using by agar well diffusion method. Volatile compounds play an important role in health care systems by the medicinal plants.
Phytochemical screening and gcms, ftir analysis of. The aim of the present study is to screen the phytochemicals, identify the compounds through gcms analysis and evaluate the toxic effect of a polyherbal drug. International journal of medicinal plants and natural products ijmpnp page 1 gcms analysis of phytochemical constituents in methanol extract of wood bark from durio zibethinus murr adeniyi s adegoke 1, oke v jerry, olatunji 2g ademola. Phytochemical screening and gcms analysis of ethanol extract. Identification of active compounds from medicinal plant. The gc ms analysis of bioactive compounds from the different extracts of the. Phytochemical evaluation and gcms analysis of hyptis verticillata.
In terms of percentage amounts 6octadecenoic acidz, 8octadecenoic acid, methyl ester, oleic acid, 9octadecenoic acidz,2hydroxy1hydroxyl methylethyl ester and nhexanedecanoic. Hyptis verticillata, phytocompounds, medicinal plants, gcms. In the present study, the gcms analysis of the ethanolic extract of plant of indigofera suffruticosa leaves showed the presence of nine compounds. Phytochemical screening and gcms analysis of leaf extract of. Plants have been an important source of medicine with qualities for thousands of years. Soxhlet extraction and liquidliquid fractionation were used to prepare different extracts and fractions from the leaves of a medicinal plant, ardisia elliptica. Pdf screening of traditionally used medicinal plants for. The gcms analysis of bioactive compounds from the different extracts of the leaves of b. Gcms analysis was carried out using a gc clarus 500 perkin elmer system comprising a aoc20i auto sampler and gas. Further studies are required to confirm the compound detected by gcms analysis. The aim of the present study is to determine the potential bioactive components of leaves of eclipta alba using gas chromatography mass spectrometry analysis gcms.
For gcms detection, an electron ionization system with ionizing energy of 70 ev was used8. Pdf gcms analysis, in vitroantioxidant and cytotoxic. Gcms analysis of methanol wild plant and callus extracts. Medicinal plants have been used for thousands of years to cure various human diseases as the plants contain many constituents which have high therapeutic.
However, the absence of scientific evaluation of medicinal plants may cause serious adverse effects. The gcms analysis was done using the instrument gcms shimadzu qp2010 with gcms solution version 2. Interpretation on mass spectrum gcms was interpretation on mass spectrum gcms was conducted using the database of national institute standard and technology nist which is having more than 62,000. Evaluation of antimicrobial potential of medicinal plants may thrive a safe, inexpensive and efficient therapeutic in developing formulation for oral care products.
Gcms study of methanolic extract of leaves of catharanthus. Methanol extract was analyzed by gcms technique in gcmsqp2010 ultra shimadzu spectrometer fitted with rtx5ms capillary column 30 m x 0. The detailed tabulations of gcms analysis of the extracts are given in table 1, table 2, table 3, table 4 and table 5 respectively. The identified compounds and their molecular formula and peak area are presented in table 2, and peaks and their rt are shown in gcms chromatogram figure 1.
Furthermore, gc ms analysis of the extract revealed. In silico docking studies were done using vlife mds 4. The methods were based on liquid chromatographytimeofflight mass spectrometry lctofms and gas chromatographymass spectrometry gcms. Gcms analysis and antimicrobial activity of hygrophila. The gcms analysis of the acetone extract revealed the presence of five major.
Two mvda methods were developed and optimised to analyse the results. Pdf phytochemical analysis of leaf extract of eclipta alba. Gcms analysed phytochemicals and antibacterial activity of. Feronia elephantum belongs to the family rutaceae is well known in indian traditional system for its traditional uses. Nov 19, 2018 a dataset of liquid chromatographymass spectrometry measurements of medicinal plant extracts from 74 species was generated and used for training and validating plant species identification. Activity of plant extractsessential oils against three plant. Chemometric profile of root extracts of rhodiola imbricata. The gcms analysis of senna alata stem revealed the presence of twenty two compounds phytochemical constituents that could contribute the medicinal quality of the plant table 2 and figure 1.
Gcms is one of the best techniques to identify the constituents of volatile matter, long chain, branched chain hydrocarbons, alcohols acids and esters etc. Gcms analysis of ethanolic extract of canthium parviflorum lamk. Gcms analysis gcms analysis was carried out in a gcms. Gcms analysis of bioactive constituents of indigofera. The compounds in the extract were quantitatively calculated using gcms concentrated around the method described by 14. Gcms analysis1l of the ethyl acetate, methanol and acetone extracts of solanum verbascifolium was used separately for the carrying out the gcms analysis for various phytochemical compounds present in the stem bark of the plant.
Frontiers gcms analysis and gastroprotective evaluations. Nov 01, 2016 the gcms analysis of bioactive compounds from the different extracts of the leaves of b. Phytochemical screening of methanolic leaves extracts revealed the presence of carbohydrates, proteins, alkaloids, phenols, flavonoids, terpenes, phytosterols, etc. Pdf gcms analysis of bioactive components of feronia. Gcms analysis of bioactive components on the leaves extract of artocarpus hirsutus. Gcms analysis of stem bark extracts of senna alata l. Gas chromatography mass spectrometry analysis 5gcms analysis of the extract was carried out using gcms clarus500 perkin elmer system and gas chromatograph interfaced to a mass spectrometer gcms employing the following conditions.
The acetone extract showed the presence of all phytoconstituents studied. India is the largest producer of medicinal herbs and is appropriately called the. The gas chromatographymass spectrometry gcms analysis of whole plant crude extract further validates the qualitative phytochemical data. The gcms gas chromatographymass spectrometry analysis of the extract. Pdf phytochemical and gcms analysis of nhexane extract of. The presence of various phytochemicals contributes many medicinal values of the plant. Gas chromatography and mass spectrum analysis gas chromatography gc analysis was carried out at advanced instrumentation research facility jnu new delhi. K the presence of phenol, flavonoid and total antioxidant in both the extract justifies the antioxidant potential of the plant which brings about its free radicals ms analysis showed the presence of 6 different phytochemicals with z 7. Gcms analysis of bioactive compounds from the whole plant. Phytochemical analysis of hybanthus enneaspermus using uv. The bioactive compounds in the ethanolic extract of s.
Phytochemical analysis by gcms analysis of the plant rhizomes revealed the presence of different fatty acids, heterocyclic. Ayurvedic herbal arrangements based on the medicinal plants are used in present health care as nutritive supplements to avoid common. Gas chromatographymass spectroscopy, a hyphenated system which is a very compatible technique and the most commonly used for the identification and quantification purpose. Evaluating in vitro antioxidant activity and gcms analysis. Gas chromatographymass spectrometry analysis 5gcms analysis of the extract was carried out using gcms clarus500 perkin elmer system and gas chromatograph interfaced to a mass spectrometer gcms employing the following conditions. Gcms analysis of bioactive phytochemicals present in ethanolic. Contribution of selected medicinal plants for cancer prevention and therapy. The major and carrying out the gcms analysis for various minor compounds with their retention time phytochemical compounds. The leaves were sequentially extracted based on the polarity viz. Gcms analysis was carried out using thermo finnigan polarisq ion trap gcms ms system comprising of an as2000 liquid.
The sample of ethanolic extract was run for 35 minutes. Volatile compound analysis using gcms, phytochemical. Gas chromatography mass spectroscopy, a hyphenated system which is a very compatible technique and the most commonly used for the identification and quantification purpose. Chemometric profile of the nhexane, chloroform, dichloroethane, ethyl acetate, methanol, and 60% ethanol root extracts of r. One such an important medicinal plant is abutilon fruticosum guill. Gcms analysis of bioactive compounds in the ethanolic extract of. Medicinal plants have been used as traditional treatments for numerous human diseases for thousands of years. The attained results of the gcms analysis of the nhexane fraction of g. Phytochemical analysis, anti inflammatory activity, in vitro. Phytochemical screening and gcms analysis were done in order to assess the chemical profiling of extract. Based on these results saponins were subjected to purification and characterization while ph. Comparison of gcms and lcms methods for the analysis of.
Several compounds were identified in gcms analysism which can be responsible for gastroprotective potentials of the plant extracts and essential oil. The aim of this study was to carry out for identification of bioactive compounds from the whole plant ethanolic extract of evolvulus alsinoides by gas chromatography and mass spectroscopy gcms. Gcms analysis of extract of leaves solanum torvum revealed the exsistance of 3nhexylthiolane, s,sdioxide 49. Gcms analysis the gc ms chromatogram of the twenty 1l of the ethyl acetate extract of one peaks of the compounds detected are phyllanthus emblica was used for the shown in the figure 3. Pdf, identification of bioactive phytochemicals using gcmass and. Spectroscopic detection by gcms involved an electron ionization system which utilized high energy electrons 70 ev. Dunal is one of the most important medicinal plants in ayurveda based medicines 1 with a wide spec. Introductionscreening of active components from plants has direct to the development of new medicinal drugs which have efficient protection and treatment role against various diseases mukhrjee, 2007. Gcms analysis of ethanolic extract was done by standard protocol using the equipment thermo gctrace ultra version. Gcms analysis of bioactive compounds present in different. Gcms analysis of methanol extract of alangium salviifolium l.
Gcms analysis of acacia nilotica twig revealed the presence of various bioactive such as limonene, stigmasterol, linoleic acid, ricinoleic acid, santalol, undecylenic acid. Zb 5 ms capillary standard nonpolar column was used. Gas chromatography mass spectrometry gc ms analysis of the whole plant. The moroccan flora is rich in medicinal plants that are. Gcms analysis gcms analysis was carried out on the ethanolic extract of canthium parviflorum and 22 compounds were identified. Pdf gcms analysis of bioactive compounds on ethanolic leaf. Plants have been an important source of medicine since ancient era.
Gcms analysis of phytoconstituents from amomum nilgiricum. For gcms detection, an electron ionization system was operated in electron impact mode. Gcms analysis of the ethanolic extract of the whole plant drosera. Phytochemicals, antioxidant and antimicrobial potentials and. Pdf characterization of some plant extracts by gcms. Phytochemical screening, gcms analysis and toxicity. The methanol extract exhibited a better antibacterial activity than others. Patel j, reddy v, kumar gs, satyasai d and krishna s.
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